Bacteria-polymer composite material for glycerol valorization

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dc.creatorRipoll, Magdalena
dc.creatorSoriano, Nicolás
dc.creatorIbarburu, Sofía
dc.creatorDalies, Malena
dc.creatorMulet, Ana Paula
dc.creatorBetancor, Lorena
dc.creator.affiliationUniversidad ORT Uruguay
dc.creator.affiliationUniversidad ORT Uruguay
dc.creator.affiliationUniversidad ORT Uruguay
dc.creator.affiliationUniversidad ORT Uruguay
dc.creator.affiliationUniversidad ORT Uruguay
dc.creator.affiliationUniversidad ORT Uruguay
dc.date.accessioned2024-05-15T19:44:23Z
dc.date.available2024-05-15T19:44:23Z
dc.date.issued2023
dc.descriptionIncluye bibliografía.
dc.description.abstractBacterial immobilization is regarded as an enabling technology to improve the stability and reusability of biocatalysts. Natural polymers are often used as immobilization matrices but present certain drawbacks, such as biocatalyst leakage and loss of physical integrity upon utilization in bioprocesses. Herein, we prepared a hybrid polymeric matrix that included silica nanoparticles for the unprecedented immobilization of the industrially relevant Gluconobacter frateurii (Gfr). This biocatalyst can valorize glycerol, an abundant by-product of the biodiesel industry, into glyceric acid (GA) and dihydroxyacetone (DHA). Different concentrations of siliceous nanosized materials, such as biomimetic Si nanoparticles (SiNps) and montmorillonite (MT), were added to alginate. These hybrid materials were significantly more resistant by texture analysis and presented a more compact structure as seen by scanning electron microscopy. The preparation including 4% alginate with 4% SiNps proved to be the most resistant material, with a homogeneous distribution of the biocatalyst in the beads as seen by confocal microscopy using a fluorescent mutant of Gfr. It produced the highest amounts of GA and DHA and could be reused for up to eight consecutive 24 h reactions with no loss of physical integrity and negligible bacterial leakage. Overall, our results indicate a new approach to generating biocatalysts using hybrid biopolymer supports.
dc.description.sponsorshipANII - FMV_1_2021_1_167184.
dc.format.extent14 p. fot., diagrs., grafs., tbls.
dc.format.mimetypePDF
dc.identifier.citationRipoll, M., Soriano N., Ibarburu, S., Dalies, M., Mulet, A. P., & Betancor, L. (2023). Bacteria-polymer composite material for glycerol valorization. Polymers, 15(11), 14 p. https://doi.org/10.3390/polym15112514
dc.identifier.doihttps://doi.org/10.3390/polym15112514
dc.identifier.urihttps://hdl.handle.net/20.500.11968/6947
dc.languageeng
dc.rights.levelAcceso abierto
dc.rights.licenseLicencia Creative Commons Atribución/Reconocimiento 4.0 Internacional (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.source.enPolymers, 15(11), 14 p.
dc.subjectHYBRID POLYMERS
dc.subjectBIOCATALYSIS
dc.subjectBACTERIAL IMMOBILIZATION
dc.subjectNANOMATERIALS
dc.subjectGLYCEROL
dc.subjectGLUCONOBACTER
dc.titleBacteria-polymer composite material for glycerol valorization
dc.typeArtículo
dc.type.versionVersión publicada
person.identifierDepartment of Biotechnology, Universidad ORT Uruguay
person.identifierFacultad de Química, Universidad de la República.
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